Crocetin suppresses the growth and migration in HCT-116 human colorectal cancer cells by activating the p-38 MAPK signaling pathway
Yousef Rasmi, Esmaeil Khajeh, Fatemeh Kheradmand, Hassan Malekinejad, Pornanong Aramwit, Ehsan Saboory, Behrokh Daeihassani, Mahdieh Nasirzadeh
Abstract
BACKGROUND AND PURPOSE: . EXPERIMENTAL APPROACH: HCT-116 cells were treated with different concentrations (0, 200, 400, 600, and 800 μM) of crocetin for 24 h. The cell survival rate was measured by MTT assay. Cell migration capacity was evaluated using the wound healing assay. The expression levels of vascular endothelial growth factor (VEGF) and matrix metalloproteinase (MMP-9) was monitored by RT-PCR. Phosphorylation of focal adhesion kinase (FAK) and p38 mitogen-activated protein kinase (MAPK) was determined using western blot. FINDINGS/RESULTS: < 0.001). However, no significant change in the phosphorylation of FAK was observed. CONCLUSION AND IMPLICATION: These data suggested that crocetin-induced growth- and migration- suppressing effects on HCT-116 cells may partially depend on the regulation of the p38 (MAPK) signaling pathway.