Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesis
Sho Tanosaki, Tomohiko Akiyama, Sayaka Kanaami, Jun Fujita, Minoru S.H. Ko, Keiichi Fukuda, Shugo Tohyama
Abstract
Here we describe a protocol to obtain highly pure cardiomyocytes and neurons from human induced pluripotent stem cells (hiPSCs) via metabolic selection processes. Compared to conventional purification protocols, this approach is easier to perform and scale up and more cost-efficient. The protocol can be applied to hiPSCs and human embryonic stem cells. For complete details on the use and execution of this protocol, please refer to Tohyama et al. (2016) and Tanosaki et al. (2020).
Topics & Concepts
Induced pluripotent stem cellHuman Induced Pluripotent Stem CellsEmbryonic stem cellStem cellCell biologyProtocol (science)ChemistryBiologyComputational biologyBiochemistryGeneMedicinePathologyAlternative medicinePluripotent Stem Cells ResearchCRISPR and Genetic EngineeringMitochondrial Function and Pathology