Faster, sharper, more precise: Automated Cluster-FLIM in preclinical testing directly identifies the intracellular fate of theranostics in live cells and tissue
Robert Brodwolf, Pierre Volz‐Rakebrand, Johannes Stellmacher, Christopher Wolff, Michael Unbehauen, Rainer Haag, Monika Schäfer‐Korting, Christian Zoschke, Ulrike Alexiev
Abstract
: The automated Cluster-FLIM tool is fitting-free, providing images with enhanced information, contrast, and spatial resolution at short exposure times and low fluorophore concentrations. Thereby, Cluster-FLIM increases the applicability of FLIM in high content analysis of target molecules in drug development and beyond.
Topics & Concepts
Fluorescence-lifetime imaging microscopyFluorescenceIntracellularHigh-content screeningLive cell imagingBiophysicsChemistryBiological systemBiologyPhysicsCellBiochemistryQuantum mechanicsAdvanced Fluorescence Microscopy TechniquesCell Image Analysis TechniquesAdvanced Biosensing Techniques and Applications