Identification of a Proline-Kinked Amphipathic α-Helix Downstream from the Methyltransferase Domain of a Potexvirus Replicase and Its Role in Virus Replication and Perinuclear Complex Formation
Ken Komatsu, Nobumitsu Sasaki, Tetsuya Yoshida, Katsuhiro Suzuki, Yuki Masujima, Masayoshi Hashimoto, Satoru Watanabe, N. Tochio, T. Kigawa, Yasuyuki Yamaji, Kenro Oshima, S. Namba, Richard S. Nelson, Tsutomu Arie
Abstract
. Substitution of the proline residue, but not the other residues necessary for replication, allowed formation of a large perinuclear complex within cells resembling those formed by PlAMV replicase and RNA during virus replication. Our results demonstrate the role of the amphipathic α-helix in PlAMV replicase in a perinuclear complex formation and virus replication and that perinuclear complex formation by the replicase alone will not necessarily indicate successful virus replication.
Topics & Concepts
BiologyRNA-dependent RNA polymeraseRNAViral replicationBiochemistryCell biologyVirologyVirusGenePlant Virus Research StudiesHIV Research and TreatmentBacteriophages and microbial interactions