Construction of a Porcine Reproductive and Respiratory Syndrome Virus with Nanoluc Luciferase Reporter: a Stable and Highly Efficient Tool for Viral Quantification Both <i>In Vitro</i> and <i>In Vivo</i>
Yang Wang, Xinna Ge, Yongning Zhang, Xin Guo, Jun Han, Lei Zhou, Hanchun Yang
Abstract
were characterized; and the results demonstrated it has greatly improved the convenience and efficiency for screening the anti-PRRSV reagents. Furthermore, a novel luciferase-based virus neutralization assay was successfully set, which can eliminate the step of sample gradient dilution and greatly improve the convenience and throughput of neutralizing antibody testing. Predictably, it will greatly facilitate the screening and evaluating anti-PRRSV therapies, as well as the mechanistic study of its replication and pathogenesis in the future.
Topics & Concepts
LuciferaseVirologyVirusBiologyTiterPorcine reproductive and respiratory syndrome virusSerial passageArterivirusCoding regionHEK 293 cellsMolecular biologyGeneticsTransfectionCell cultureGeneMedicineInfectious disease (medical specialty)Coronavirus disease 2019 (COVID-19)PathologyDiseaseAnimal Virus Infections StudiesVirus-based gene therapy researchViral gastroenteritis research and epidemiology