Alginate hydrogel polymers enable efficient delivery of a vascular-targeted AAV vector into aortic tissue
Anca Remes, Dima Ibrahim Basha, Thomas Puehler, Christopher Borowski, Susanne Hille, Laura Kummer, Andreas H. Wagner, Markus Hecker, Jasmin Soethoff, Georg Lutter, Derk Frank, Rawa Arif, Norbert Frey, Marcin Zaradzki, Oliver J. Müller
Abstract
into AAV9SLR-containing hydrogel, and efficient transduction could be confirmed. These findings demonstrate that alginate hydrogel harboring a vascular-targeting AAV9SLR vector allows efficient local transduction of the aortic wall.
Topics & Concepts
Transduction (biophysics)Gene deliveryAdventitiaViral vectorAortaSelf-healing hydrogelsCell biologyGenetic enhancementEx vivoAdeno-associated virusVascular smooth muscleIn vivoChemistryMedicineBiomedical engineeringBiologyPathologyVector (molecular biology)Recombinant DNAGeneBiochemistrySmooth muscleInternal medicineBiotechnologyOrganic chemistryRNA Interference and Gene DeliveryVirus-based gene therapy researchTissue Engineering and Regenerative Medicine