Preparation and characterization of biomimetic gradient multi-layer cell-laden scaffolds for osteochondral integrated repair
Mingxin Li, Ping Song, Wenzhao Wang, Xu Yang, Jun Li, Lina Wu, Xingyu Gui, Zhimou Zeng, Zhigang Zhou, Ming Liu, Qingquan Kong, Yujiang Fan, Xingdong Zhang, Changchun Zhou, Lei Liu
Abstract
experiments confirmed that cells were distributed homogeneously and that different pore structures could affect the proliferation and differentiation of BMSCs. The Nonporous hydrogel was beneficial for the chondrogenic differentiation of BMSCs and interconnected pores were conducive to BMSC proliferation and osteogenic differentiation. The osteochondral integrative repair capacity of the scaffold was assessed by implanting the scaffolds into the intercondylar defect of the rabbit femur. By constructing pore structures in different layers, the cells in different layers of the hydrogels were in an intrinsic environment for survival and differentiation. Animal experiments confirmed that tissue engineering scaffolds for osteochondral lesions require different pore structures in different layers, and gradient structure facilitated integrated repair.