Abnormal overexpression of G9a in melanoma cells promotes cancer progression via upregulation of the Notch1 signaling pathway
Ningning Dang, Jing Jiao, Xianguang Meng, Yunhe An, Han Chen, Shuhong Huang
Abstract
. Next, we treated cells with UNC0642, and observed a significant decrease in cell viability in M14 and A375 cell lines. Furthermore, treatment with UNC0642 resulted in increased apoptosis. In immunocompetent mice bearing A375 engrafts, treatment with UNC0642 inhibited tumor growth. Results of Western blot analysis revealed that administration of UNC0642 or silencing of G9a expression by siRNA reduced Notch1 expression significantly and decreased the level of Hes1 in A375. All in all, the data from our study demonstrates potential of G9a as a therapeutic target in the treatment of melanoma.
Topics & Concepts
Cancer researchMelanomaViability assayDownregulation and upregulationBiologyGene silencingCell growthCancerApoptosisHistone methyltransferaseCancer cellCarcinogenesisCell cultureSkin cancerChemistryEpigeneticsBiochemistryGeneticsGeneEpigenetics and DNA MethylationHistone Deacetylase Inhibitors ResearchGenomics and Chromatin Dynamics