Large-Scale CRISPRi and Transcriptomics of Staphylococcus epidermidis Identify Genetic Factors Implicated in Lifestyle Versatility
Michelle Spoto, Johanna P. Riera Puma, Elizabeth Fleming, Changhui Guan, Yvette Ondouah Nzutchi, Dean Kim, Julia Oh
Abstract
Staphylococcus epidermidis is a bacteria that broadly inhabits healthy human skin, yet it is also a common cause of skin infections and bloodstream infections associated with implanted medical devices. Because human skin has many different types of S. epidermidis, each containing different genes, our goal is to determine how these different genes allow S. epidermidis to switch from healthy growth in the skin to being an infectious pathogen. Understanding this switch is critical to developing new strategies to prevent and treat S. epidermidis infections.
Topics & Concepts
Staphylococcus epidermidisBiologyTranscriptomeComputational biologyCRISPR interferenceMetabolomicsMicrobiomeGenetic screenMicrobial metabolismGeneticsBioinformaticsGeneCRISPRBacteriaGene expressionPhenotypeCas9Staphylococcus aureusCRISPR and Genetic EngineeringBacterial biofilms and quorum sensingMicrobial Metabolism and Applications