Rapid antibiotic susceptibility testing using resazurin bulk modified screen-printed electrochemical sensing platforms
Benjamin Crane, Jack P. Hughes, Samuel J. Rowley‐Neale, Mamun Rashid, Patricia Linton, Craig E. Banks, Kirsty J. Shaw
Abstract
(ATCC® 25922) to the antibiotic gentamicin sulphate using DPV to detect the relative concentrations of resazurin between antibiotic treated bacteria, and bacteria without antibiotic treatment. Using R-SPEs, antibiotic susceptibility was determined after a total elapsed time of 90 minutes including the inoculation of the artificial urine, preincubation and testing time. The use of electrochemistry as a phenotypic means of identifying an effective antibiotic to treat a complicated UTI offers a rapid and accurate alternative to culture based methods for AST with R-SPEs offering an inexpensive and simpler alternative to other AST methods utilising electrochemical based approaches.