Pathogenesis Study of Enterovirus 71 Using a Novel Human SCARB2 Knock-In Mouse Model
Yuefei Jin, Tiantian Sun, Guangyuan Zhou, Dong Li, Shuaiyin Chen, Weiguo Zhang, Xueyuan Li, Rongguang Zhang, Haiyan Yang, Guangcai Duan
Abstract
Enterovirus 71 (EV71) is associated with severe hand-foot-mouth disease. Recently, outbreaks of EV71 infection with high mortality have been reported in the Asia-Pacific region, posing a great challenge for global public health. To date, the precise mechanism of EV71-induced disease, particularly the neuropathogenesis and respiratory disorders, is still not fully understood because no suitable animal models are available. Human scavenger receptor class B, member 2 (hSCARB2), has been identified as a cellular receptor for EV71. Here, we introduce a novel CRISPR/Cas9-mediated hSCARB2 knock-in (KI) mouse model for the study of EV71 pathogenesis, which is of great significance for the development of antiviral drugs and vaccines.