An improved pathway for autonomous bioluminescence imaging in eukaryotes
Ekaterina S. Shakhova, Tatiana A. Karataeva, Nadezhda M. Markina, Tatiana Mitiouchkina, Kseniia A. Palkina, Maxim M. Perfilov, Monika G. Wood, Trish T. Hoang, Mary P. Hall, Liliia I. Fakhranurova, Anna E. Alekberova, Alena K. Malyshevskaia, D.A. Gorbachev, Evgenia N. Bugaeva, Ludmila K. Pletneva, Vladislav V. Babenko, Daria I. Boldyreva, Andrey Yu. Gorokhovatsky, Anastasia V. Balakireva, Feng Gao, V. V. Choob, Lance P. Encell, Keith V. Wood, Ilia V. Yampolsky, Karen S. Sarkisyan, Alexander S. Mishin
Abstract
The discovery of the bioluminescence pathway in the fungus Neonothopanus nambi enabled engineering of eukaryotes with self-sustained luminescence. However, the brightness of luminescence in heterologous hosts was limited by performance of the native fungal enzymes. Here we report optimized versions of the pathway that enhance bioluminescence by one to two orders of magnitude in plant, fungal and mammalian hosts, and enable longitudinal video-rate imaging.