Structure elucidation of the redox cofactor mycofactocin reveals oligo-glycosylation by MftF
Luis de la Peña, Ana Patrícia Graça, Huijuan Guo, Daniel Braga, Tobias G. Köllner, Lars Regestein, Christine Beemelmanns, Gerald Lackner
Abstract
-methylglucose. Dissection of biosynthetic genes demonstrated that the oligoglycosylation is catalyzed by the glycosyltransferase MftF. Furthermore, we confirm the redox cofactor function of glycosylated MFTs by activity-based metabolic profiling using the carveol dehydrogenase LimC and show that the MFT pool expands during cultivation on ethanol. Our results will guide future studies into the biochemical functions and physiological roles of MFT in bacteria.
Topics & Concepts
GlycosylationRedoxCofactorChemistryStereochemistryBiochemistryOrganic chemistryEnzymeMicrobial metabolism and enzyme functionMetal-Catalyzed Oxygenation MechanismsEnzyme Structure and Function