Isolation, cultivation, and application of primary respiratory epithelial cells obtained by nasal brushing, polyp samples, or lung explants
Anita Golec, Iwona Pranke, Paolo Scudieri, Kate Hayes, Elise Dréano, Fiona Dunlevy, Aurélie Hatton, D.G. Downey, Luis J. V. Galietta, Isabelle Sermet‐Gaudelus
Abstract
Here, we present a standardized protocol for isolation, maintenance, and polarization of the respiratory epithelial primary cells from patient samples acquired from nasal brushing, polyp specimens, or lung explants. This protocol generates a clearly defined polarized layer of epithelial cells on filters, with a good number of ciliated cells and a thin layer of mucus. We detail the steps for samples prepared from patients with cystic fibrosis as well as from subjects without cystic fibrosis.
Topics & Concepts
Cystic fibrosisExplant cultureLungRespiratory systemMucusIsolation (microbiology)Respiratory tractPathologyMedicineBiologyMicrobiologyInternal medicineIn vitroEcologyBiochemistryNeonatal Respiratory Health ResearchTracheal and airway disorders3D Printing in Biomedical Research