Circulating <scp>microRNA</scp> breast cancer biomarker detection in patient sera with surface plasmon resonance imaging biosensor
Chi Lok Wong, Sau Yeen Loke, Hann Qian Lim, Ghayathri Balasundaram, Patrick Chan, Bee Kiang Chong, Ern Yu Tan, Ann S. G. Lee, Malini Olivo
Abstract
In this article, we report for the first time, the detection of circulating miRNA as a breast cancer biomarker in patient sera using surface plasmon resonance imaging biosensor. The advantage of this approach lies in the rapid, label-free and sensitive detection. The sensor excites plasmonic resonance on the gold sensor surface and specific DNA-miRNA molecular bindings elucidate responses in the plasmonic resonance image. Experiments of detecting synthetic miRNA molecules (miR-1249) were performed and the sensor resolution was found to be 63.5 nM. The sensor was further applied to screen 17 patient serum samples from National Cancer Centre Singapore and Tan Tock Seng Hospital. Sensor intensity response was found to differ by 20% between malignant and benign cases and thus forms, a potential and an important metric in distinguishing benignity and malignancy.