Activity-Based Protein Profiling of Retaining α-Amylases in Complex Biological Samples
Yurong Chen, Zachary Armstrong, Marta Artola, Bogdan I. Florea, C.J. Kuo, Casper de Boer, Mikkel S. Rasmussen, Maher Abou Hachem, Gijsbert A. van der Marel, Jeroen D. C. Codée, Johannes M. F. G. Aerts, G.J. Davies, Herman S. Overkleeft
Abstract
-cyclophellitol-based pseudodisaccharides equipped with a suite of reporter entities and their use in ABPP of retaining amylases from human saliva, murine tissue as well as secretomes from fungi grown on starch. The activity and efficiency of the inhibitors and probes are substantiated by extensive biochemical analysis, and the selectivity for amylases over related retaining endoglycosidases is validated by structural studies.
Topics & Concepts
AmylaseChemistryStarchBiochemistryEnzymeSalivaComputational biologyBiologyEnzyme Production and CharacterizationEnzyme Catalysis and ImmobilizationPeptidase Inhibition and Analysis