Visible light-activatable Q-dye molecular beacons for long-term mRNA monitoring in neurons
Robin Klimek, Paul Donlin-Asp, Claudio Polisseni, Vanessa Hanff, Erin M. Schuman, Alexander Heckel
Abstract
Herein, we present a new class of Q-dye molecular beacons (MBs) that can be locally activated with visible light in hippocampal neurons. Our novel architecture increases the available monitoring time for neuronal mRNA from several minutes to 14 hours, since a lower light-sampling rate is required for tracking.
Topics & Concepts
Molecular beaconBeaconVisible spectrumChemistryMessenger RNAHippocampal formationSampling (signal processing)BiophysicsBiologyMaterials scienceOptoelectronicsBiochemistryComputer scienceGeneNeuroscienceReal-time computingTelecommunicationsDetectorOligonucleotideLuminescence and Fluorescent MaterialsPhotochromic and Fluorescence ChemistryPhotoreceptor and optogenetics research