Synergism of silver/CEM drug on novel proteinaceous silk fibroin/mesoporous silica based sandwich-layered nanofibrous scaffolds for osteoregenerative applications
M. Rama, U. Vijayalakshmi
Abstract
Osteoregeneration and bacterial infection remains a major challenge in current clinical practice despite, many advanced strategies significantly developed so far. The combination of bioactive molecules and nano topology design can favour osteoinducivity that holds a greater potential as a bone substitute and minimize implant failure due to burst antibacterial effect . Here, we have reported a novel design of sandwich-like layered silver doped mesoporous silica nanofibers stuffed with an antibiotic drug combined with silk fibroin to boost the synergistic effect . We have investigated the physiochemical property of CEM incorporated sandwiched scaffolds by in vitro biomineralization, tensile strength , antibacterial efficacy against Escherichia coli and Staphylococcus aureus bacterial strains, in vitro degradation, osteogenic effects (MTT assay using MG63 cell line and ARS staining). In conclusion, the faster degradation of monolayered nanofibrous scaffold by incorporating the sandwich technique was minimized. The combination of CEM/SF embedded Ag@mesoporous silica sandwich layered nanofibers showed a synergistic antibacterial effect due to the sustained release of drug from the middle layer with no burst release. The development of sandwich technique for the nanofibrous scaffolds could be a viable candidate for bone regeneration and defect repair.