A Transposon-Associated CRISPR/Cas9 System Specifically Eliminates both Chromosomal and Plasmid-Borne <i>mcr-1</i> in Escherichia coli
Yuzhang He, Jin-Ru Yan, Bing He, Hao Ren, Xu Kuang, Tengfei Long, Caiping Chen, Xiao‐Ping Liao, Yahong Liu, Jian Sun
Abstract
The global spread of antimicrobial-resistant bacteria has been one of the most severe threats to public health. The emergence of the mcr-1 gene has posed a considerable threat to antimicrobial medication since it deactivates one last-resort antibiotic, colistin.
Topics & Concepts
MCR-1ColistinPlasmidEscherichia coliCRISPRBiologyTransposable elementAntimicrobialGeneticsMicrobiologyCas9BacteriaEscherichia coli ProteinsAntibioticsGeneEnterobacteriaceaeGenomeAntibiotic Resistance in BacteriaCRISPR and Genetic EngineeringBacterial Genetics and Biotechnology