Isolation, Identification and Antimicrobial Resistance Profiles of Salmonella from Dairy Farms in Adama and Modjo Towns, Central Ethiopia
Abdurezak Abrar, Takele Beyene, Walkite Furgasa, K Alexander, L Warnick, M Wiedmannm, Alam Md, Md Gahangir, Sezanur Uddin, Tasnim Rahman, Mohammad Ahmad, Pulak Shakil Mahmood, Md Maitra, A Ayalu, B Seyoum, J Yimam, T Beyene, B Tesega, T Beyene, H Yibeltie, B Chebo, F Abunna, A Beyi, B Mammo, D Ayana, R Duguma, T Callaway, J Keen, T Edrington, L Baumgard, K Vanamburgh, T Poole, R Harvey, D Nisbet, A Demissie, T Wubie, F Yehuala, A Fluit, Fufa Abunna, Debebe Ashenafi, Takele Beyene, Dinka Ayana, Bedaso Mamo, Reta Duguma, M Guibourdenche, P Roggentin, M Mikoleit, I Fields, J Bockemuhl, P Grimont, F Weill, A Happy, M Alam, S Mahmud, M Islam, M Ahmed, M Uddin, S Hoffmann, M Batz, J Morris, H Karin, I Andrea, Martin, J Kemal, M Kirk, S Pires, R Black, H Kusumaningrum, G Riboldi, W Hazeleger, R Beumer, A Mathew, R Cissell, S Liamthong, Z Mccuddin, S Carlson, M Rasmussen, S Franklin, Md, Pulak Uddin, H Maitra, Md Faruquee, Alam, W Molla, B Molla, D Alemayehu, A Muckle, L Cole, E Wilkie, E Ponce, A Khan, C Cheng, W Summage, C Cerniglia, C Pui, W Wong, L Chai, M Rahman, A Haque, T Ahmad
Abstract
A cross-sectional study was carried out from February 2019 to May 2019 in Adama and Modjo aiming at isolating Salmonella from dairy cattle farms and determining the antimicrobial susceptibility testing of the isolates. A total of 117 samples from dairy farms: faces, bulk tank milk, personnel hand swab, and contaminated floor samples were collected and screened for the presence of Salmonella. Ten (8.5%) of the samples tested were found to be positive for Salmonella. Of 89 faces, 10 bulk tank milk, 9 personnel hand swab, and 9 contaminated floor samples, no positive was found in the milker’s hand swab samples from both Adama and Modjo areas and the isolation frequencies of Salmonella were 8.98%, 10%, and 11.1% in faces, bulk tank milk, and floor sample, respectively. The antibiogram testing revealed differential multi-drug resistance among Salmonella isolates in lactating cow and cows environment samples. Most of the isolates were resistant to methicillin, streptomycin, and nalidixic acid whereas sensitivity was recorded for gentamicin. In conclusion, the relatively high resistance among the bacteria present in dairy farms could pose public health and therapeutic problems to consumers as potential vehicles of resistant Salmonella foodborne infections.