Anti-RAFLS Triterpenoids and Hepatoprotective Lignans From the Leaves of Tujia Ethnomedicine Kadsura heteroclita (Xuetong)
Mengyun Wang, Sai Jiang, Nusrat Hussain, Salman Zafar, Qingling Xie, Feibing Huang, Linxi Mao, Bin Li, Yuqing Jian, Wei Wang
Abstract
A pair of 3,4- seco -cycloartane triterpenoid isomers with a rare peroxy bridge, namely, xuetonins A and B ( 1 and 2 ), four new lignans xuetonlignans A–D ( 3 – 6 ), a new sesquiterpene xuetonpene ( 7 ), and a new natural product xuetonin C ( 8 ), along with 43 known compounds, were obtained from the leaves of Tujia ethnomedicine, Kadsura heteroclita . Their structures and configurations were determined with the help of a combination of 1D- and 2D-NMR, HRESIMS spectra, electronic circular dichroism (ECD), and X-ray diffraction data. Compounds 2 , 10 , 13 – 15 , and 17 – 19 showed moderate-to-potent activity against rheumatoid arthritis fibroblast-like synoviocytes (RAFLS) with IC 50 values of 19.81 ± 0.26, 12.73 ± 0.29, 5.70 ± 0.24, 9.25 ± 0.79, 5.66 ± 0.52, 11.91 ± 0.44, 13.22 ± 0.27, and 15.94 ± 0.36 μM, respectively. Furthermore, compounds 22 , 25 , and 31 exhibited significant hepatoprotective effects against N -acetyl- p -aminophenol (APAP)–induced toxicity in HepG2 cells at 10 μM, and the cell viability increased by 12.93, 25.23, and 13.91%, respectively, compared with that in the model group (cf. bicyclol, 12.60%).