MicroED: conception, practice and future opportunities
Max T. B. Clabbers, Anna Shiriaeva, Tamir Gonen
Abstract
This article documents a keynote seminar presented at the IUCr Congress in Prague, 2021. The cryo-EM method microcrystal electron diffraction is described and put in the context of macromolecular electron crystallography from its origins in 2D crystals of membrane proteins to today's application to 3D crystals a millionth the size of that needed for X-ray crystallography. Milestones in method development and applications are described with an outlook to the future.
Topics & Concepts
Context (archaeology)Computer scienceData scienceNanotechnologyEngineering ethicsChemistryElectron crystallographyElectron diffractionLibrary scienceWorld Wide WebPolitical scienceDiffractionEngineeringEngineering physicsEnzyme Structure and FunctionAdvanced Electron Microscopy Techniques and ApplicationsProtein Structure and Dynamics