CRISPR/Cas12a-mediated gold nanoparticle aggregation for colorimetric detection of SARS-CoV-2
Yiren Cao, Jinjun Wu, Bo Pang, Hongquan Zhang, X. Chris Le
Abstract
The trans-cleavage activity of the target-activated CRISPR/Cas12a liberated an RNA crosslinker from a molecular transducer, which facilitated the assembly of gold nanoparticles. Integration of the molecular transducer with isothermal amplification and CRISPR/Cas12a resulted in visual detection of the N gene and E gene of SARS-CoV-2 in 45 min.
Topics & Concepts
CRISPRColloidal goldNanoparticleRNASevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Cleavage (geology)Coronavirus disease 2019 (COVID-19)ChemistryNanotechnology2019-20 coronavirus outbreakCombinatorial chemistryVirologyBiologyMaterials scienceBiochemistryInfectious disease (medical specialty)GeneMedicineFracture (geology)PathologyOutbreakDiseasePaleontologyCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesAdvanced Nanomaterials in Catalysis