Reduced growth rate of aged muscle stem cells is associated with impaired mechanosensitivity
Mohammad Haroon, Heleen E. Boers, Astrid D. Bakker, Niek G.C. Bloks, Willem M.H. Hoogaars, Lorenzo Giordani, René J.P. Musters, Louise Deldicque, Katrien Koppo, Fabien Le Grand, Jenneke Klein‐Nulend, Richard T. Jaspers
Abstract
were substantially downregulated. When subjected to pulsating fluid shear stress, aged MuSCs exhibited reduced upregulation of proliferation-related genes. Together these results indicate that aged MuSCs exhibit impaired mechanosensitivity and growth potential, accompanied by altered morphology and mechanical properties as well as reduced integrin-α7 expression. Aging-associated impaired muscle regenerative capacity and muscle wasting is likely due to aging-induced intrinsic MuSC alterations and dysfunctional mechanosensitivity.