Portable Differential Detection of CTX-M ESBL Gene Variants, <i>bla</i> <sub>CTX-M-1</sub> and <i>bla</i> <sub>CTX-M-15</sub> , from Escherichia coli Isolates and Animal Fecal Samples Using Loop-Primer Endonuclease Cleavage Loop-Mediated Isothermal Amplification
Owen Higgins, Alexandra Chueiri, Louise O’Connor, Sinéad Lahiff, Liam P. Burke, Dearbháile Morris, Nicola Maria Pfeifer, Belén González-Santamarina, Christian Berens, Christian Menge, Manuela Caniça, Vera Manageiro, Veljo Kisand, Marwa M. Hassan, Brian Gardner, Arnoud H. M. van Vliet, Roberto M. La Ragione, Bruno González‐Zorn, Terry Smith
Abstract
, facilitating improved identification and surveillance of these closely related variants. Further application of this portable internally controlled multiplex CTX-M-1/15 LEC-LAMP assay will provide new information on the transmission and prevalence of these CTX-M ESBL alleles. Furthermore, this transferable diagnostic technology can be applied to other new and emerging relevant AMR markers of interest providing more efficient and specific portable pathogen detection for improved epidemiological surveillance.