A novel minimal motif for left-handed G-quadruplex formation
Poulomi Das, Fernaldo Richtia Winnerdy, Arijit Maity, Yves Méchulam, Anh Tuân Phan
Abstract
A recent study on the left-handed G-quadruplex (LHG4) DNA revealed a 12-nt minimal motif GTGGTGGTGGTG with the ability to independently form an LHG4 and to drive an adjacent sequence to LHG4 formation. Here we have identified a second LHG4-forming motif, GGTGGTGGTGTG, and determined the X-ray crystal structure of an LHG4 involving this motif. Our structural analysis indicated the role of split guanines and single thymine loops in promoting LHG4 formation.
Topics & Concepts
Motif (music)G-quadruplexStructural motifThymineStereochemistryChemistryCrystallographyDNAPhysicsBiochemistryAcousticsDNA and Nucleic Acid ChemistryAdvanced biosensing and bioanalysis techniquesDNA and Biological Computing