The Dynll1-Cox4i1 Complex Regulates Intracellular Pathogen Clearance via Release of Mitochondrial Reactive Oxygen Species
Jiangbei Yuan, Zihan Zheng, Liting Wang, Haiying Ran, Xiangyu Tang, Xiaodong Xie, Fei Li, Fang Liu, Xiaoyang Wang, Jiale Zhang, Junying Zhang, Yi Huang, Xuefeng Xia, Ying Wan
Abstract
Cellular membrane proteins are a critical part of the host defense mechanisms against infection and intracellular survival of Listeria monocytogenes . The complex spatiotemporal regulation of bacterial infection by various membrane proteins has been challenging to study. Here, using mass spectrometry analyses, we depicted the dynamic expression landscape of membrane proteins upon L. monocytogenes infection in dendritic cells. We showed that Dynein light chain 1 (Dynll1) formed a persistent complex with the mitochondrial cytochrome oxidase Cox4i1, which is disturbed by pathogen insult.