Cre/ <i>lox</i> -Mediated CRISPRi Library Reveals Core Genome of a Type I Methanotroph <i>Methylotuvimicrobium buryatense</i> 5GB1C
Minggen Cheng, Dongmei Pei, Lian He, Qiang Fei, Xin Yan
Abstract
Due to their key role in methane cycling and their industrial potential, methanotrophs have drawn increasing attention. Genome-wide experimental approaches for gene-phenotype mapping accelerate our understanding and engineering of a bacterium. However, these approaches are still unavailable in methanotrophs. This work has two significant implications. First, the core genes identified here provide functional genetic basics for complete reconstruction of the metabolic network and afford more clues for knowledge gaps. Second, the Cre-mediated knock-in method developed in this work enables large-scale DNA library construction in methanotrophs; the CRISPRi library can be used to screen the genes associated with special culture conditions.