The Role of Intestinal Microbiota and Mast Cell in a Rat Model of Visceral Hypersensitivity
Yingjie Li, Jing Li, Cong Dai
Abstract
Background/Aims: To explore the role of intestinal flora and mast cells in visceral hypersensitivity (VH). Methods: The experimental animals were divided into 4 groups: control group, VH group, VH + VSL#3 group, and VH + ketotifen group. Stool samples were collected from each group (n = 3) for a further analysis using 16S ribosomal DNA gene sequence. Visceral sensitivity was evaluated by abdominal withdrawal reflex (AWR) score. Colon tissues of rats were obtained from each group. Mast cells were detected by toluidine blue staining. The degranulation of mast cells was assessed by transmission electron microscopy. Results: 1 abundance was higher in the VH group compared to the control group, which could be restored by application of probiotic VSL#3. Conclusions: 1 abundance may be a basis for VH observed in irritable bowel syndrome and may be prevented by specific probiotic administration.