In situ studies of membrane biology by cryo-electron tomography
Jenny Keller, Rubén Fernández‐Busnadiego
Abstract
Cryo-electron tomography (cryo-ET) allows high resolution 3D imaging of biological samples in near-native environments. Thus, cryo-ET has become the method of choice to analyze the unperturbed organization of cellular membranes. Here, we briefly discuss current cryo-ET workflows and their application to study membrane biology in situ, under basal and pathological conditions.
Topics & Concepts
Cryo-electron tomographyBiologyIn situElectron tomographyCryo-electron microscopyMembraneBiophysicsTomographyCell biologyResolution (logic)Electron microscopeArtificial intelligenceComputer scienceBiochemistryPhysicsOpticsScanning transmission electron microscopyMeteorologyAdvanced Electron Microscopy Techniques and ApplicationsLipid Membrane Structure and BehaviorNanopore and Nanochannel Transport Studies