Real-time imaging of protein microenvironment changes in cells with rotor-based fluorescent amino acids
Shudan Yang, Shikai Jin, Mengxi Zhang, Yuda Chen, Yiming Guo, Yu Hu, Peter G. Wolynes, Han Xiao
Topics & Concepts
Amino acidFluorescenceChemistryBiochemistryFluorescent proteinCell biologyChemical biologyBiophysicsGreen fluorescent proteinProtein structureProtein foldingProtein–protein interactionHEK 293 cellsProtein engineeringStructural biologyProtein biosynthesisBimolecular fluorescence complementationFluorescent labellingComputational biologyProteomeFörster resonance energy transferYellow fluorescent proteinBiologyPlasma protein bindingPeptide sequenceDissociation (chemistry)Target proteinGenetically engineeredFluorescence-lifetime imaging microscopyFusion proteinTumor microenvironmentMolecular imagingRNA and protein synthesis mechanismsRNA Research and SplicingUbiquitin and proteasome pathways