Near-Complete Genomes of Two <i>Trichoderma</i> Species: A Resource for Biological Control of Plant Pathogens
Yi Zhou, Yilian Wang, Kai Chen, Yuanzheng Wu, Jindong Hu, Yanli Wei, Jishun Li, Hetong Yang, Maarten H. Ryder, Matthew D. Denton
Abstract
Trichoderma species are widely used to control fungal and nematode diseases of crops. To date, only one complete Trichoderma genome has been sequenced, T. reesei QM6a, a model fungus for industrial enzyme production, while the species or strains used for biological control of plant diseases are only available as draft genomes. Previously, we demonstrated that two Trichoderma strains (T. afroharzianum and T. cyanodichotomus) provide effective control of nematode and fungal plant pathogens. Based on deep sequencing using Illumina and Pacbio platforms, we have assembled high-quality genomes of the above two strains, with contig N 50 reaching 4.2 and 1.7 Mbp, respectively, which is greater than those of published draft genomes. The genome data will provide a resource to assist research on the biological control mechanisms of Trichoderma spp.