Phosphotyrosine-based Phosphoproteomics for Target Identification and Drug Response Prediction in AML Cell Lines
Carolien van Alphen, Jacqueline Cloos, Robin Beekhof, David G. J. Cucchi, Sander R. Piersma, Jaco C. Knol, Alex A. Henneman, Thang V. Pham, Johan van Meerloo, Gert J. Ossenkoppele, Henk M.W. Verheul, Jeroen J. W. M. Janssen, Connie R. Jiménez
Abstract
internal tandem duplication (ITD) mutation.Our data show the potential of pY-phosphoproteomics and INKA analysis to provide insight in AML TK signaling and identify hyperactive kinases as potential targets for treatment in AML cell lines. These results warrant future investigation of clinical samples to further our understanding of TK phosphorylation in relation to clinical response in the individual patient.
Topics & Concepts
PhosphoproteomicsCancer researchTyrosine kinaseReceptor tyrosine kinaseKinaseCell cultureFms-Like Tyrosine Kinase 3PDGFRAMyeloid leukemiaMyeloidBiologyMutationSignal transductionCell biologyBiochemistryProtein kinase AGeneticsProtein phosphorylationStromal cellGeneGiSTAcute Myeloid Leukemia ResearchAdvanced Proteomics Techniques and ApplicationsMultiple Myeloma Research and Treatments