Leveraging Cas13a's <i>trans</i>-cleavage on RNA G-quadruplexes for amplification-free RNA detection
Tao Li, Dongjuan Chen, Xiaoling He, Zheyu Li, Zhichen Xu, R.Y. Li, Bingxin Zheng, Rui Hu, Jiang Zhu, Ying Li, Yunhuang Yang
Abstract
-cleaving RNA G-quadruplexes (rG4s) as an alternative to ssRNA reporters in CRISPR-Cas13a diagnostics. Our findings demonstrate enhanced efficiency due to the structural arrangement of rG4s. Implementing a simplified CRISPR-Cas13a system based on rG4, we identified SARS-CoV-2 infections in 25 patient samples within 1 hour without target pre-amplification.
Topics & Concepts
RNACleavage (geology)ChemistryMolecular biologyComputational biologyBiologyGeneticsGeneFracture (geology)PaleontologyCRISPR and Genetic EngineeringRNA and protein synthesis mechanismsAdvanced biosensing and bioanalysis techniques