ALIBY: ALFA Nanobody-Based Toolkit for Imaging and Biochemistry in Yeast
Dipayan Akhuli, Anubhav Dhar, Aileen Sara Viji, Bindu Bhojappa, Saravanan Palani
Abstract
In yeast research, conventional fluorescent protein tags and small epitope tags are widely used to study the spatiotemporal dynamics and activity of proteins. Although proven to be efficient, these tags lack the versatility for use across different cell biological and biochemical studies of a given protein of interest.
Topics & Concepts
YeastSaccharomyces cerevisiaePlasmidBiologyComputational biologyCell biologyLive cell imagingBiochemistryCellGeneAdvanced Electron Microscopy Techniques and ApplicationsRNA and protein synthesis mechanismsBiotin and Related Studies