Reovirus σ1 Conformational Flexibility Modulates the Efficiency of Host Cell Attachment
Julia R. Diller, Sean R. Halloran, Melanie Koehler, Rita dos Santos Natividade, David Alsteens, Thilo Stehle, Terence S. Dermody, Kristen M. Ogden
Abstract
Nonenveloped virus entry is an incompletely understood process. For reovirus, the functional significance of conformational rearrangements in the attachment protein, σ1, that occur during entry and particle uncoating are unknown. We engineered and characterized reoviruses containing cysteine mutations that cross-link σ1 monomers in nonreducing conditions. We found that the introduction of a cysteine pair in the receptor-binding domain of σ1 yielded a virus that replicates with faster kinetics than the parental virus and forms larger plaques. Using functional assays, we found that cross-linking the σ1 receptor-binding domain modulates reovirus attachment but not uncoating or transcription. These data suggest that σ1 conformational rearrangements mediate the efficiency of reovirus host cell binding.