Evaluation of the Performance of a Multiplexed Serological Assay in the Detection of SARS-CoV-2 Infections in a Predominantly Vaccinated Population
Michael Asamoah-Boaheng, David A. Goldfarb, Vilte Barakauskas, Tracy L Kirkham, Paul A. Demers, Mohammad Ehsanul Karim, Pascal M. Lavoie, Ana Márquez, Agatha N. Jassem, Sandra Jenneson, Christopher MacDonald, Brian Grunau
Abstract
Among a cohort of adult paramedics in Canada, we investigated the performance of nucleocapsid (N) antibody detection (measured with a V-PLEX assay) to identify previous COVID-19 infections and compared differences among vaccinated and unvaccinated. Our data indicate that vaccinated and unvaccinated groups require different thresholds to achieve optimal test performance, especially for detecting COVID-19 within the preceding 9 months. Overall, specificity was significantly higher among unvaccinated, compared to vaccinated individuals.