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SourceFinder: a Machine-Learning-Based Tool for Identification of Chromosomal, Plasmid, and Bacteriophage Sequences from Assemblies

Derya Aytan-Aktug, Vladislav Grigorjev, Judit Szarvas, Philip T. L. C. Clausen, Patrick Munk, Marcus Nguyen, James J. Davis, Frank M. Aarestrup, Ole Lund

2022Microbiology Spectrum18 citationsDOIOpen Access PDF

Abstract

Extra-chromosomal genes encoding antimicrobial resistance, metal resistance, and virulence provide selective advantages for bacterial survival under stress conditions and pose serious threats to human and animal health. These accessory genes can impact the composition of microbiomes by providing selective advantages to their hosts. Accurately identifying extra-chromosomal elements in genome sequence data are critical for understanding gene dissemination trajectories and taking preventative measures. Therefore, in this study, we developed a random forest classifier for identifying the source of bacterial chromosomal, plasmid, and bacteriophage sequences.

Topics & Concepts

BacteriophagePlasmidIdentification (biology)Computational biologyBiologyGeneticsComputer scienceArtificial intelligenceGeneBotanyEscherichia coliBacteriophages and microbial interactionsGenomics and Phylogenetic StudiesBacterial Genetics and Biotechnology
SourceFinder: a Machine-Learning-Based Tool for Identification of Chromosomal, Plasmid, and Bacteriophage Sequences from Assemblies | Litcius