An optimized protocol for generating appendage-bearing skin organoids from human-induced pluripotent stem cells
I. Ahmed, Jane Sun, Jason Brown, Kiarash Khosrotehrani, Abbas Shafiee
Abstract
Abstract Organoid generation from pluripotent stem cells is a cutting-edge technique that has created new possibilities for modelling human organs in vitro, as well as opening avenues for regenerative medicine. Here, we present a protocol for generating skin organoids (SKOs) from human-induced pluripotent stem cells (hiPSCs) via direct embryoid body formation. This method provides a consistent start point for hiPSC differentiation, resulting in SKOs with complex skin architecture and appendages (e.g. hair follicles, sebaceous glands, etc.) across hiPSC lines from two different somatic sources.
Topics & Concepts
OrganoidInduced pluripotent stem cellEmbryoid bodyRegenerative medicineDirected differentiationStem cellBiologyCell biologyHuman Induced Pluripotent Stem CellsSomatic cellAnatomyEmbryonic stem cellGeneticsGenePluripotent Stem Cells ResearchCellular Mechanics and Interactions3D Printing in Biomedical Research