Imaging neuronal migration and network activity in human forebrain assembloids
Fikri Birey, Sergiu P. Pașca
Abstract
Assembloids generated from human pluripotent stem cells are self-organizing, multicellular in vitro models that recapitulate aspects of cell-cell interactions and circuit assembly during neural development. Here, we present protocols to functionally monitor, in forebrain assembloids, the migration of GABAergic interneurons from the ventral to the dorsal forebrain and the activity in early cortical networks. Specifically, we describe high-resolution imaging and analysis of neuronal migration as well as calcium imaging of network activity in forebrain assembloids. For complete details on the use and execution of this protocol, please refer to Birey et al. (2022).
Topics & Concepts
ForebrainNeuroscienceCalcium imagingMulticellular organismBiologyGABAergicInduced pluripotent stem cellCellEmbryonic stem cellChemistryCalciumCentral nervous systemOrganic chemistryInhibitory postsynaptic potentialBiochemistryGeneticsGeneNeurogenesis and neuroplasticity mechanismsNeuroscience and Neural EngineeringCell Image Analysis Techniques