Significantly enhancing production of <i>trans</i> -4-hydroxy- <scp>l</scp> -proline by integrated system engineering in <i>Escherichia coli</i>
Mengfei Long, Meijuan Xu, Zhenfeng Ma, Xuewei Pan, Jiajia You, Mengkai Hu, Yu Shao, Taowei Yang, Xian Zhang, Zhiming Rao
Abstract
-4-hydroxy-l-proline in 60 hours almost without l-proline remaining-the highest production to date. The de novo engineering carbon flux through rare codon selected evolution, dynamic precursor modulation, and metabolic engineering provides a good technological platform for efficient hydroxyl amino acid synthesis.
Topics & Concepts
ProlineHydroxylationEscherichia coliFermentationBiochemistryChemistryFlux (metallurgy)Amino acidEnzymeOrganic chemistryGeneAmino Acid Enzymes and MetabolismMicrobial Metabolic Engineering and BioproductionEnzyme Structure and Function