A simple and rapid colorimetric detection of serum lncRNA biomarkers for diagnosis of pancreatic cancer
Ut Kei Lou, Chi Hin Wong, Yangchao Chen
Abstract
A colorimetric assay is developed for detection of lncRNA HOTTIP by one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) coupled with positively-charged gold nanoparticles ((+)AuNP) for diagnosis of pancreatic cancer. This assay allows simple, rapid, and sensitive quantification of lncRNA down to 50 copies.
Topics & Concepts
Pancreatic cancerSimple (philosophy)ChemistryColorimetryCancer researchCancerChromatographyInternal medicineMedicineEpistemologyPhilosophyCancer-related molecular mechanisms researchMicroRNA in disease regulationCancer Genomics and Diagnostics