Miniprep assisted proteomics (MAP) for rapid proteomics sample preparation
C. Bruce Mousseau, Camille Pierre, Daniel D. Hu, Matthew M. Champion
Abstract
existing approaches and reproducibility of replicate minipreps was 98.8% at the 1% FDR protein level. Label Free Quantitative proteomics was performed and 91.2% of quantified proteins had a %CV <20% (2044/2241). Miniprep Assisted Proteomics can be performed in minutes, shows low variability, high recovery and proteome depth. This suggests a significant role for adventitious binding in developing new proteomics sample preparation techniques. MAP represents an efficient, ultra-low-cost alternative for sample preparation in a commercially obtainable device that costs ∼$0.50 (USD) per miniprep.
Topics & Concepts
ProteomicsSample preparationConsistency (knowledge bases)Sample (material)Computer scienceChromatographyComputational biologyChemistryBiologyArtificial intelligenceBiochemistryGeneAdvanced Proteomics Techniques and ApplicationsAdvanced Biosensing Techniques and ApplicationsGlycosylation and Glycoproteins Research