Bioethanol Production from Date Seed Cellulosic Fraction Using Saccharomyces cerevisiae
Fatma Abdelhedi, Amal Ben Abdeddayem, Mohamed Koubaa, Francisco J. Barba, Khawla Ben Jeddou, Imen Kacem, Raoudha Ellouz Ghorbel, Semia Ellouz Châabouni
Abstract
This study investigates the feasibility of producing ethanol from date palm seeds. The chemical compositions of three varieties of date seeds were first studied, showing mainly the presence of cellulose and hemicellulose. Ethanol was produced after a pre-treatment of date seeds using acid hydrolysis to extract the cellulosic fraction and to remove the lignin. Producing ethanol by fermentation was performed using the yeast Saccharomyces cerevisiae for 24 h, during which ethanol yield, biomass concentration, and total reducing sugars were recorded. The results obtained showed that the sugar content decreased over time, while ethanol production increased. Indeed, date seeds gave the highest ethanol concentration of 21.57 g/L after 6 h of alcoholic fermentation. These findings proved the feasibility of producing ethanol from date seeds.