Biodegradation of Bisphenol A in a saline industrial wastewater using Alcaligenes faecalis strain BPAN5
Abdolkazem Neisi, Alireza Rahmani, Shokouh Ghafari, Sahand Jorfi, Halime Almasi
Abstract
ABSTRACT A large portion of petrochemical wastewaters are classified as high total dissolved solids, containing bisphenol A (BPA) which makes them resistant to conventional degradation processes. In this work, a halotolerant bacterial isolate, Alcaligenes faecalis strain BPAN5 was isolated and used for biodegradation of BPA. Effect of operational parameters including initial seed size (5–20 mL), nitrogen source, salinity (2%–3.5%) and BPA concentrations (10–60 mg/L) on BPA removal was investigated. Results showed that the addition of NH 4 Cl in the culture medium led to the highest BPA biodegradation rate and bacterial growth. The most BPA removal of 100% and 96% were observed for the initial seed size of 15 mL and salinity of 2% for BPA concentrations of 10 and 20 mg/L, respectively. The main metabolites of BPA biodegradation were chlorobenzene, chlorothymol, hexadecanoic acid and 2,6-di-tert-butyl-4-phenylphenol. Also, the biodegradability of effluent reached 69% while the toxicity decreased to 7%. Findings of current work indicated the significant ability of Alcaligenes faecalis strain BPAN5 for biodegradation of BPA in a saline medium.