A hairpin probe-mediated exponential amplification reaction for highly sensitive and specific detection of microRNAs
Hongyan Yu, Zhi Weng, Xi Zhou, Dan Bai, Wang Luo, Xiaole Han, Lin Song, Qian Liu, Junjie Li, Yujun Yang, Yongcan Guo, Ke Lv, Guoming Xie
Abstract
In this work, we propose a hairpin probe-mediated exponential amplification reaction (HEAR) strategy that combines DNA strand displacement with a "who triggers, who gets generated" mode, providing excellent single-base discrimination and a reduced background signal. The detection limit is 19 aM, which is reduced by 3 orders of magnitude compared to traditional exponential amplification approaches. This one-pot strategy also exhibits a wide dynamic range, high specificity and short detection time. It is expected to become a powerful tool for clinical diagnosis.
Topics & Concepts
Exponential functionMultiple displacement amplificationDNAmicroRNASIGNAL (programming language)ChemistryBiophysicsBiological systemPhysicsBiologyPolymerase chain reactionComputer scienceMathematicsGeneBiochemistryMathematical analysisDNA extractionProgramming languageAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene DeliveryDNA and Nucleic Acid Chemistry