Site-Selective and Rewritable Labeling of DNA through Enzymatic, Reversible, and Click Chemistries
Andrew A. Wilkinson, Elodie Jagu, Krystian Ubych, Steven Coulthard, Ashleigh E. Rushton, Jack Kennefick, Qiang Su, Robert K. Neely, Francisco Fernández‐Trillo
Abstract
DNA is exploited to sequentially introduce two fluorescent dyes on DNA. Finally, we demonstrate the potential of the method by developing a protocol to sort labeled DNA using magnetic beads, with subsequent amplification of the sorted DNA sample for further analysis. The presented method opens new avenues for site-selective bioconjugation and should underpin integrative approaches in chemical biology where sequential functionalizations of the same sample are required.
Topics & Concepts
BioconjugationClick chemistryHydrazoneCombinatorial chemistryDNAChemistryChemical biologyHydrazideBiomoleculeLinkerComputer scienceBiochemistryStereochemistryOrganic chemistryProgramming languageClick Chemistry and ApplicationsAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene Delivery