Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Kenneth J. Livak, Thomas D. Schmittgen
Topics & Concepts
Real-time polymerase chain reactionQuantitative analysis (chemistry)DeltaComputational biologySample (material)BiologyBiological systemComputer scienceMolecular biologyGeneChromatographyChemistryGeneticsPhysicsAstronomyMolecular Biology Techniques and ApplicationsRNA Research and SplicingGene expression and cancer classification