Quantitative Top-Down Proteomics in Complex Samples Using Protein-Level Tandem Mass Tag Labeling
Dahang Yu, Zhe Wang, Kellye A. Cupp‐Sutton, Yanting Guo, Qiang Kou, Kenneth Smith, Xiaowen Liu, Si Wu
Abstract
cell lysates. To our knowledge, this represents the first high-throughput TMT labeling-based, quantitative, top-down MS analysis suitable for complex biological samples.
Topics & Concepts
ChemistryIsobaric labelingTandem mass tagTandem mass spectrometryProteomicsMass spectrometryChromatographyTop-down proteomicsQuantitative proteomicsTandemSample preparationSelected reaction monitoringProtein mass spectrometryBiochemistryMaterials scienceGeneComposite materialAdvanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and ApplicationsBiosensors and Analytical Detection