Glucose Metabolism and Acetate Switch in Archaea: the Enzymes in Haloferax volcanii
Tom Kuprat, Marius Ortjohann, Ulrike Johnsen, Peter Schönheit
Abstract
In this work, we provide a comprehensive analysis of glucose degradation via the semiphosphorylative Entner-Doudoroff pathway in the haloarchaeal model organism Haloferax volcanii . The study includes transcriptional analyses, growth experiments with deletion mutants. and characterization of all enzymes involved in the conversion of 3-phosphoglycerate to acetyl coenzyme A (acetyl-CoA) and in anaplerosis.
Topics & Concepts
Haloferax volcaniiBiochemistryBiologyAcetate kinaseEnzymeCofactorArchaeaEscherichia coliGeneEnzyme Structure and FunctionAmino Acid Enzymes and MetabolismMetabolism and Genetic Disorders